文章基本信息

标题：Specification of Positional Information in Retinal Ganglion Cells of Xenopus: Assays for Analysis of the Unspecified State
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作者：R. K. Hunt ; Marcus Jacobson
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1973
卷号：70
期号：2
页码：507-511
DOI：10.1073/pnas.70.2.507
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The central connections of retinal ganglion cells are retinotopically organized, producing a "map" of the retina on the surface of the optic tectum. Exactly how and when individual ganglion cells develop the position-dependent properties (termed locus specificities) subserving formation of the map is unknown, but the positional information that each ganglion cell will use in this process is specified in the early Xenopus embryo during a critical period at stages 28-32. We report two methods for isolating eye primordia from the axial cues of the animals during this critical period and for then allowing the eyes to form retinotectal connections in a carrier embryo. The results show that, as early as optic vesicle stages 22-23, the eyes already contain orthogonal reference axes, that positional information can be specified with respect to these axes in vitro, and that the specification process itself may only entail a transition from a reversible to an irreversible state.
关键词：neuronal specificity ; retinotectal connections ; tissue culture ; serial eye transplantation