文章基本信息

标题：Hapten-Conjugated Antibodies and Visual Markers Used to Label Cell-Surface Antigens for Electron Microscopy: An Approach to Double Labeling
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作者：Michael E. Lamm ; Gloria C. Koo ; Christopher W. Stackpole 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1972
卷号：69
期号：12
页码：3732-3736
DOI：10.1073/pnas.69.12.3732
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Haptens can be coupled to mouse immunoglobulin with retention of antibody specificity. Such hapten-coupled alloantibody, when bound to cells, can be bridged to an electron microscopic marker in two ways: (i) by a hybrid antibody, i.e., anti-hapten:anti-marker, and (ii) by untreated bivalent anti-hapten antibody if the marker is also haptenized. Through the use of immunologically non-crossreactive haptens and markers it should be possible to localize multiple, different antigens on the surface of an individual cell. Double labeling has been achieved with the first method.
关键词：cell membranes ; histocompatibility antigens ; hybrid antibodies