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标题：Binding of Thymus- and Bone Marrow-Derived Lymphoid Cells to Antigen-Derivatized Fibers
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作者：U. Rutishauser ; G. M. Edelman
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1972
卷号：69
期号：12
页码：3774-3778
DOI：10.1073/pnas.69.12.3774
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Thymus-derived lymphocytes (T cells) and bone marrow-derived lymphocytes (B cells) from mouse spleens bind specifically to antigen-derivatized nylon fibers. The fiber-bound population consisted of about 60%-70% B cells and 30% T cells as determined by cytotoxicity, fluorescence, and antibody-complement binding assays. Essentially all fiber-bound cells were viable and could be accounted for as T or B cells. Enriched populations of T or B cells could be isolated on the fibers by destruction of one or the other cell type with the appropriate antiserum plus complement. T or B cells could also be fractionated according to their relative affinity (or avidity) for a given antigen.
关键词：mouse spleen ; nylon fibers ; antiserum ; cytotoxicity ; fluorescence