文章基本信息

标题：Resolution and Reconstitution of the Phosphatidate-Synthesizing System of Rat-Liver Microsomes
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作者：Satoshi Yamashita ; Kohei Hosaka ; Shosaku Numa 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1972
卷号：69
期号：11
页码：3490-3492
DOI：10.1073/pnas.69.11.3490
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The phosphatidate-synthesizing system of rat-liver microsomes was resolved into two component enzymes, glycerolphosphate acyltransferase and 1-acylglycerolphosphate acyltransferase. The resolution is effected by sucrose density gradient centrifugation in the presence of a nonionic detergent, Triton X-100. Combination of both enzymes results in reconstitution of the phosphatidate-synthesizing system. These results establish that two distinct enzymes, glycerolphosphate acyltransferase and 1-acylglycerolphosphate acyltransferase, are required for synthesis of phosphatidic acid from sn-glycerol 3-phosphate. Furthermore, the 1-acylglycerolphosphate acyltransferase preparation efficiently uses unsaturated (or saturated) fatty acyl-CoA as acyl donor. Our previous studies showed that the glycerolphosphate acyltransferase preparation catalyzes formation of 1-acylglycerol 3-phosphate, using preferentially saturated fatty acyl-CoA as acyl donor. These findings indicate that the reconstituted system is capable of yielding phosphatidic acid with an asymmetric fatty acid distribution.
关键词：Triton X-100 ; sucrose density gradient centrifugation ; glycerolphosphate acyltransferase ; 1-acylglycerolphosphate acyltransferase ; asymmetric fatty acid distribution