文章基本信息

标题：Specificity of the Break Produced by Restricting Endonuclease R1 in Simian Virus 40 DNA, as Revealed by Partial Denaturation Mapping
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作者：Carel Mulder ; Hajo Delius
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1972
卷号：69
期号：11
页码：3215-3219
DOI：10.1073/pnas.69.11.3215
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Superhelical circular (form I) SV40 DNA was converted to linear molecules by the action of a partially purified restriction enzyme of Resistance Transfer Factor-R1 of Escherichia coli. The resulting linear DNA molecules are full length, as judged by their sedimentation through alkaline sucrose gradient and by direct observation in an electron microscope. Nicked circular (form II) DNA was found as an intermediate in the conversion of form I DNA to linear DNA. Analysis of partial denaturation maps obtained by alkaline denaturation of the unitlength linear molecules showed that the break in SV40 DNA occurred at a specific site on the DNA.
关键词：tumor viruses ; electron microscopy ; resistance transfer factor R₁