文章基本信息

标题：In Vitro Repair of X-Irradiated DNA Extracted from Bacillus subtilis Deficient in Polymerase I
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作者：Philip J. Laipis ; A. T. Ganesan
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1972
卷号：69
期号：11
页码：3211-3214
DOI：10.1073/pnas.69.11.3211
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：DNA extracted from x-irradiated cells of a mutant of Bacillus subtilis deficient in DNA polymerase I has greatly reduced biological activity. This DNA, which has many single-strand nicks, can be repaired in vitro with purified DNA polymerase I and DNA ligase or with lysates of wild-type B. subtilis cells, which restore biological activity and increase the single-strand molecular weight. Lysates of polymerase I-deficient B. subtilis cells cannot repair such irradiated DNA until purified DNA polymerase I or lysate from wild-type cells is added to the deficient lysate.
关键词：DNA ligase ; gene repair ; transformation