文章基本信息

标题：Membrane Site Modified on Induction of the Transformation of Lymphocytes by Periodate
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作者：Abraham Novogrodsky ; Ephraim Katchalski
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1972
卷号：69
期号：11
页码：3207-3210
DOI：10.1073/pnas.69.11.3207
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Lymphocytes isolated from different sources undergo blastogenesis after mild treatment with periodate. Incubation of mouse spleen lymphocytes with neuraminidase or papain markedly reduces their response to periodate. Transformation induced by concanavalin A of the enzymically treated cells is not impaired. Blastogenesis induced by periodate is decreased when periodate-treated lymphocytes are reacted with borohydride, hydroxylamine, or semicarbazide. Treatment with these compounds does not affect transformation of cells by concanavalin A. It is suggested that different membrane sites are involved in lymphocyte transformation induced by periodate and by concanavalin A. The periodate target site seems to include a glycoprotein complex containing sialic acid that yields, on oxidation, an aldehyde moiety that is essential for transformation.
关键词：mouse spleen ; concanavalin A ; neuraminidase ; papain