文章基本信息

标题：Nucleic Acid Hybridization with RNA Immobilized on Filter Paper
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作者：W. C. Saxinger ; C. Ponnamperuma ; D. Gillespie 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1972
卷号：69
期号：10
页码：2975-2978
DOI：10.1073/pnas.69.10.2975
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：RNA has been immobilized in a manner suitable for use in molecular hybridization experiments with dissolved RNA or DNA by a nonaqueous solid-phase reaction with carbonyldiimidazole and RNA "dry coated" on cellulose or, preferably, on previously activated phosphocellulose filters. Immobilization of RNA does not appear to alter its chemical character or cause it to acquire affinity for unspecific RNA or DNA. The versatility and efficiency of this method make it potentially attractive for use in routine analytical or preparative hybridization experiments, among other applications.
关键词：carbonyldiimidazole coupling ; E. coli RNA ; fibroblast cell RNA ; cellulose