文章基本信息

标题：Differences in the Binding of Fluorescent Concanavalin A to the Surface Membrane of Normal and Transformed Cells
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作者：Jacob Shoham ; Leo Sachs
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1972
卷号：69
期号：9
页码：2479-2482
DOI：10.1073/pnas.69.9.2479
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The binding of fluorescein-conjugated Concanavalin A to the cell surface has been studied in normal and transformed cells in interphase and mitosis. Binding to the cell surface was in the form of an incomplete ring of fluorescence, and the binding was inhibited by [α]-methyl-D-mannopyranoside. All the cells were fluorescent when treated with 25-250 {micro}g/ml of fluorescent Concanavalin A. With 10 {micro}g, the cells were all fluorescent after 30 min of binding, but after 0.5-5 min with 10 {micro}g or 30 min with 1 or 2.5 {micro}g, transformed interphase cells showed a higher percentage of cells with surface fluorescence than did normal interphase cells. Trypsinized normal and transformed interphase cells showed the same fluorescence. Binding with 2.5 {micro}g at 4{degrees} instead of at 24{degrees
关键词：lectins ; hamster ; mouse 3T3 cells ; agglutination ; mitosis