文章基本信息

标题：In Vitro Complementation as an Assay for New Proteins Required for Bacteriophage T4 DNA Replication: Purification of the Complex Specified by T4 Genes 44 and 62
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作者：Jack Barry ; Bruce Alberts
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1972
卷号：69
期号：9
页码：2717-2721
DOI：10.1073/pnas.69.9.2717
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：We have developed an in vitro complementation assay for six T4 bacteriophage gene products believed to be components of the T4 DNA replication apparatus. This assay is based upon the fact that DNA synthesis in an infected cell lysate that lacks a given gene product is specifically stimulated by addition of the missing product. By the use of such an assay, two proteins that appear to be the products of T4 genes 44 and 62 have been purified to electrophoretic homogeneity as a single complex of the two polypeptide chains.
关键词：gene products ; mutants ; cell lysates