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  • 标题:Glycerol as an Enzyme-Stabilizing Agent: Effects on Aldehyde Dehydrogenase
  • 本地全文:下载
  • 作者:Shelby L. Bradbury ; William B. Jakoby
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:1972
  • 卷号:69
  • 期号:9
  • 页码:2373-2376
  • DOI:10.1073/pnas.69.9.2373
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:The potassium-dependent aldehyde dehydrogenase (EC 1.2.1.3 ), from yeast is markedly altered by the addition of high concentrations of glycerol or other polyhydric alcohols to aqueous buffers. Several lines of evidence suggest that the three-dimensional structure near the active site is involved: (i) The stability of the enzyme when stored at 2{degrees
  • 关键词:thiol groups ; polyhydric alcohols ; arsenite
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