文章基本信息

标题：Excretion of Deoxyribonucleic Acid by Lymphocytes Stimulated with Phytohemagglutinin or Antigen
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作者：John C. Rogers ; David Boldt ; Stuart Kornfeld 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1972
卷号：69
期号：7
页码：1685-1689
DOI：10.1073/pnas.69.7.1685
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：When human lymphocytes are cultured in the presence of phytomitogens, 70-90% of the cells undergo blast transformation and synthesize DNA. However, less than 40% of these lymphocytes actually undergo mitosis while 35-90% of the newly synthesized DNA is excreted into the media. The release of DNA by the cells is selective since experiments with [14C]uridine indicate that RNA is not lost into the culture media. DNA excretion occurs under many culture conditions. The excreted DNA has an estimated molecular weight of 3 to 12 x 106 as determined by gel filtration on Sepharose 2B. It forms a single sharp peak at a density of 1.055 g/cm3 when examined by sucrose density gradient centrifugation, suggesting that the DNA is complexed to protein or lipid.
关键词：mitogenesis ; pulse labeling ; density gradient centrifugation ; DNase