文章基本信息

标题：Identification of Lactate Dehydrogenase Isoenzymes by Rapid Kinetics
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作者：Michael J. Bishop ; Johannes Everse ; Nathan O. Kaplan 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1972
卷号：69
期号：7
页码：1761-1765
DOI：10.1073/pnas.69.7.1761
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Stopped-flow kinetics indicate that human and chicken heart-type4 lactate dehydrogenases (LDH) become inhibited as DPNH is oxidized in the presence of high concentrations of pyruvate. This inhibition is much less marked with the human and chicken muscle-type4 enzymes. The initial rates and the difference in inhibition between the two types of enzyme have made it possible to determine the amount of, as well as the ratio between, the two types of LDH that are present in a given sample by a single kinetic assay. The stopped-flow kinetic method has been used to analyze amounts of LDH isoenzyme in different tissues, as well as in serum.
关键词：stopped-flow technique ; serum enzymes ; ternary complex