文章基本信息

标题：A Molecular Theory of Ion-Conducting Channels: A Field-Dependent Transition Between Conducting and Nonconducting Conformations
本地全文：下载
作者：D. W. Urry
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1972
卷号：69
期号：6
页码：1610-1614
DOI：10.1073/pnas.69.6.1610
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Structural and conformational requirements for an electric field-dependent transition between conducting and nonconducting macromolecular systems are: two kinetically interconvertible and energetically similar conformations, one conducting and the other nonconducting, which have axes spanning the lipid layer of biological membranes, but which have different net dipole moments along those axes. Two examples are described. A previously defined helix, the {pi}6LD-helix now termed the {beta}63,3-helix, is proposed as the conducting species, and the linear peptide correlate of the cyclic hexapeptide conformation containing two {beta}-turns and an inversion element of symmetry is proposed as a nonconducting species. The latter is termed an anti-{beta}62-spiral and contains little or no net dipole moment per turn, whereas the {beta}63,3-helix contains a net dipole moment along the helix axis of about 0.5 Debye per dipeptide unit. A related conducting and nonconducting pair with large net dipole moments of opposite sign, termed syn-{beta}62-spiral and {beta}62,4-helix, are also described. The spiral conformations are stabilized in a lipid layer by intermolecular hydrogen bonds, leading to a linear association of transmembrane structures. A conformational transition in one member of the array could lead to destabilization of an adjacent member of the array. The conformational analysis uses a concept of cyclic conformations with linear conformational correlates. The anti-{beta}62-spiral and {beta}63,3-helix are derivable from the conformations of the cyclic structure [unk], whereas the syn-{beta}2-spiral and {beta}62,4-helix may be derived from the cyclic structure [unk]. The conformational analysis leads to the expectation that N-formyl-(L-Ala-L-Ala-Gly)n would form conducting channels.
关键词：biological membranes ; gramicidin A ; dipole moments ; electric field