文章基本信息

标题：Measurement of Transmembrane Potentials in Phospholipid Vesicles
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作者：Roger D. Kornberg ; Mark G. McNamee ; Harden M. McConnell 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1972
卷号：69
期号：6
页码：1508-1513
DOI：10.1073/pnas.69.6.1508
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Phosphatidylcholine vesicles are permeable to tempotartrate, a spin-label derivative of tartaric acid. The inside-outside distribution of tempotartrate is coupled to the inside-outside distribution of H+, so it must be a measure of the transmembrane electrical potential difference in vesicles permeable to H+. This prediction is borne out by the finding that the inside-outside distribution of tempotartrate is the reciprocal of the inside-outside distribution of K+ in vesicles prepared in the presence of valinomycin. The inside-outside distribution of tempotartrate is, by contrast, equal to the inside-outside distribution of Cl- in vesicles without valinomycin. This is evidence that an inside-outside Cl- concentration gradient induces an H+ gradient, which must be due to HCl permeation.
关键词：spin labels ; valinomycin