文章基本信息

标题：Lac Repressor Binding to Synthetic DNAs of Defined Nucleotide Sequence
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作者：A. D. Riggs ; S. Lin ; R. D. Wells 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1972
卷号：69
期号：3
页码：761-764
DOI：10.1073/pnas.69.3.761
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Binding of lac repressor to 20 synthetic DNAs of high molecular weight with defined repeating sequences was investigated by competition experiments. Although none of these DNAs binds repressor as tightly as does lac operator, most do bind to a measurable extent. Their affinity for repressor varies greatly and is a function of both nucleotide composition and sequence. Poly(dC-dC){middle dot}poly(dG-dC) competes for repressor 200-times less well than either poly(dA-dT){middle dot}poly(dA-dT) or poly(dT-dT-dG){middle dot}poly(dC-dA-dA). The other DNAs show a broad spectrum of affinities for repressor between these extremes. These results show that the lac repressor has affinity for, and can distinguish between, sequences distantly related to its operator.
关键词：nearest-neighbor analysis ; rate competition experiments ; equilibrium dissociation constants