文章基本信息

标题：Affinity Chromatography of RNA-Dependent DNA Polymerase from RNA Tumor Viruses on a Solid Phase Immunoadsorbent
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作者：David M. Livingston ; Edward M. Scolnick ; Wade P. Parks 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1972
卷号：69
期号：2
页码：393-397
DOI：10.1073/pnas.69.2.393
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：A solid phase immunoadsorbent specific for RNA-dependent DNA polymerase from murine and feline RNA tumor viruses has been prepared. The enzymes from murine and feline virus but not from avian virus bind to columns of this material. Bound enzymes can be eluted in active form. This method has permitted selective purification of viral enzyme from crude extracts of virus-transformed cells, since the immunoadsorbent has no affinity for cellular DNA polymerases.
关键词：murine leukemia virus ; feline sarcoma-leukemia virus complex ; Rous sarcoma virus