文章基本信息

标题：Synthesis of DNA in Toluene-Treated Chlamydomonas reinhardi
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作者：Stephen H. Howell ; Linda L. Walker
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1972
卷号：69
期号：2
页码：490-494
DOI：10.1073/pnas.69.2.490
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Toluene-treated Chlamydomonas reinhardi incorporate deoxynucleoside triphosphates (dNTPs) into DNA. Incorporation requires all four dNTPs, but requires neither ATP nor added DNA. The incorporation reaction is linear for nearly 20 min. The product of synthesis in treated cells is mainly chloroplast ({beta}-component) DNA. However, a small quantity of nuclear ([α]-component) DNA is also synthesized. Synchronously grown cells are most efficient in dNTP incorporation at a time in the cell cycle when chloroplast DNA is normally replicated. Toluene treatment disrupts the internal morphology of the cell, but leaves the outer membrane of the chloroplast intact.
关键词：DNA replication ; chloroplast DNA ; cell cycle ; electron microscopy