文章基本信息

标题：Direct Evidence for a Prostaglandin Receptor and Its Application to Prostaglandin Measurements
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作者：F. A. Kuehl ; J. L. Humes
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1972
卷号：69
期号：2
页码：480-484
DOI：10.1073/pnas.69.2.480
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：A prostaglandin receptor is present in rat adipocytes, as shown by competition studies with [3H]-prostaglandin E1. The affinity of the E prostaglandins for this receptor preparation is greater than that of A and F prostaglandins, an observation consistent with their relative potencies in stimulating cyclic AMP formation in isolated mouse ovaries and various other organs. In the protocol described, binding constants of 3-8 nM were obtained with prostaglandins E2 and E1. A sensitivity of 1 pmole makes this method readily applicable for measurement of tissue concentrations of the E prostaglandins.
关键词：rat ; adipocytes ; antagonists ; analogues ; mouse ovary assay