文章基本信息

标题：Inhibition of Release of Dopamine-β-Hydroxylase and Norepinephrine from Sympathetic Nerves by Colchicine, Vinblastine, or Cytochalasin-B
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作者：Nguyen B. Thoa ; G. Frederick Wooten ; Julius Axelrod 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1972
卷号：69
期号：2
页码：520-522
DOI：10.1073/pnas.69.2.520
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Stimulation of the hypogastric nerve to the guinea pig vas deferens in the presence of phenoxybenzamine produced an enhanced release of both dopamine-{beta}-hydroxylase (EC 1.14.2.1 ) and norepinephrine. Addition of colchicine, vinblastine, or cytochalasin-B to the incubation medium caused an almost complete inhibition of release of both the amine and the enzyme. Colchicine and cytochalasin-B produced a blocking effect but did not, however, modify the ratio of dopamine-{beta}-hydroxylase to norepinephrine recovered in the bath fluid. The findings indicate that norepinephrine and dopamine-{beta}-hydroxylase are released from sympathetic nerve terminals by exocytosis and that this process is dependent upon the integrity of both microtubules and microfilaments.
关键词：hypogastric nerve stimulation ; exocytosis ; microtubules ; microfilaments ; guinea pig