文章基本信息

标题：Detection of Avian Tumor Virus-Specific Nucleotide Sequences in Avian Cell DNAs
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作者：Harold E. Varmus ; Robin A. Weiss ; Robert R. Friis 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1972
卷号：69
期号：1
页码：20-24
DOI：10.1073/pnas.69.1.20
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The effect of unlabeled cellular DNA upon the reassociation kinetics of labeled double-stranded DNA made by DNA polymerase from avian tumor viruses has been used to measure virus-specific nucleotide sequences in cells. Multiple copies of these sequences were found equally in normal chick cells, in chick cells transformed in culture, and in Rous tumor cells. Copies were also present equally in cells harboring chick helper factor and group-specific antigen, and in cells lacking those characteristics. Quail DNA also contains multiple copies of these sequences, but no copies were detected in HeLa-cell DNA or salmon-sperm DNA.
关键词：reassociation kinetics ; RNA tumor viruses ; gs antigen ; Rous sarcoma virus ; chick cells