文章基本信息

标题：Mechanism of Action of Bacitracin: Complexation with Metal Ion and C55-Isoprenyl Pyrophosphate
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作者：K. John Stone ; Jack L. Strominger
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1971
卷号：68
期号：12
页码：3223-3227
DOI：10.1073/pnas.68.12.3223
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The inhibition by bacitracin of the enzymatic dephosphorylation of C55-isoprenyl pyrophosphate is abolished by the addition of chelating agents. If, however, the chelating agent is added after a preincubation of bacitracin with a divalent cation and the lipid substrate, then its addition has little effect, indicating that bacitracin, metal ion, and C55-isoprenyl pyrophosphate form a complex. Various divalent cations can participate in complex formation, but monovalent cations are ineffective. A direct demonstration of the formation of a complex between the C55-isoprenyl pyrophosphate and bacitracin in the presence of metal ions was obtained. Molecular models that show one possible conformation for a complex between bacitracin and the C55-isoprenyl pyrophosphate, in which the metal ion acts as a bridge between the two compounds, are presented.
关键词：molecular model ; chelating agents ; ternary complex ; cell wall synthesis ; peptidoglycan