文章基本信息

标题：A Complex Between Initiation Factor IF2, Guanosine Triphosphate, and fMet-tRNAf: An Intermediate in Initiation Complex Formation
本地全文：下载
作者：Arthur H. Lockwood ; Prasanta R. Chakraborty ; Umadas Maitra 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1971
卷号：68
期号：12
页码：3122-3126
DOI：10.1073/pnas.68.12.3122
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Evidence is presented that suggests the formation of a complex between polypeptide chain-initiation factor IF 2, GTP, and fMet-tRNAf. This complex transfers both fMet-tRNAf and GTP to 30S ribosomal subunits in the presence of ApUpG and initiation factor IF 1. The resultant 30S initiation complex reacts with 50S subunits to form a 70S initiation complex. fMet-tRNAf in this 70S complex reacts with puromycin to form fMet-puromycin. These results suggest that [IF 2, GTP, fMet-tRNAf] is an intermediate in the initiation of protein synthesis in Escherichia coli.
关键词：E. coli ; protein biosynthesis ; 30S ribosomal subunits ; Sephadex filtration ; 70S ribosomal complex