文章基本信息

标题：Products of RNA Polymerases in HeLa Cell Nuclei
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作者：Ester A. Zylber ; Sheldon Penman
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1971
卷号：68
期号：11
页码：2861-2865
DOI：10.1073/pnas.68.11.2861
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：RNA polymerase activities in intact HeLa cell nuclei have been examined and compared to activities investigated in previous studies of the purified enzymes. The RNA synthesized by the mammalian polymerases while still in nuclei is identified. The polymerases are tentatively identified by location, sensitivity to [α]-amanitin, and response to manganese and altered ionic strength. Polymerase I is located in the nucleolus and labels partially complete precursor molecules of ribosomal RNA. Polymerases II and III are in the nucleoplasm and both label giant nuclear heterogeneous RNA.
关键词：α-amanitin ; actinomycin D ; ionic strength