文章基本信息

标题：RNA Polymerases of Maize: Nuclear RNA Polymerases
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作者：Gustave C. Strain ; Kathleen P. Mullinix ; Lawrence Bogorad 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1971
卷号：68
期号：11
页码：2647-2651
DOI：10.1073/pnas.68.11.2647
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Two DNA-dependent RNA polymerases of nuclear origin have been purified from leaves of Zea mays. The two enzymes can be separated on DEAE-cellulose columns. Enzymes I and II are eluted with 0.08 and 0.20 M (NH4)2SO4, respectively. Both enzymes prefer maize nuclear DNA as a template; they are also more active in the presence of Mg++ than Mn++ and are inhibited by (NH4)2-SO4 or KCl. Neither enzyme is inhibited by rifamycin SV. Enzyme II is strongly inhibited by [α]-amanitin, whereas enzyme I is not significantly affected. Their ability to use native and denatured DNA as templates varies according to the extent and method of purification of the polymerase. Furthermore, enzyme II can be resolved by DEAE-chromatography or glycerol-gradient centrifugation into two components, one of which prefers native DNA, while the other prefers denatured DNA.
关键词：Zea mays ; rifamycin SV ; α-amanitin ; native and denatured DNA ; Mg⁺⁺ ; Mn⁺⁺