文章基本信息

标题：Inactivation of D-Alanine Carboxypeptidase by Penicillins and Cephalosporins Is Not Lethal in Bacillus subtilis
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作者：Peter M. Blumberg ; Jack L. Strominger
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1971
卷号：68
期号：11
页码：2814-2817
DOI：10.1073/pnas.68.11.2814
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The D-alanine carboxypeptidase of Bacillus subtilis is a particulate enzyme that is irreversibly inactivated by penicillins and cephalosporins. However, the lethal concentrations of these antibiotics are not the same as those that inhibit enzymatic activity in vitro. 6-Aminopenicillanic acid inactivates at least 95% of the enzyme at nonlethal concentrations. Conversely, cephalothin is lethal at concentrations that do not inactivate the enzyme. Experiments with intact, growing cells confirm the results obtained in vitro. Therefore, a killing site distinct from the carboxypeptidase must be postulated.
关键词：peptidoglycan ; mucopeptide ; cell wall ; 6-aminopenicillanic acid ; cephalothin ; cloxacillin