文章基本信息

标题：Lateral Diffusion of Phospholipids in a Vesicle Membrane
本地全文：下载
作者：Roger D. Kornberg ; Harden M. McConnell
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1971
卷号：68
期号：10
页码：2564-2568
DOI：10.1073/pnas.68.10.2564
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Nuclear-resonance spectra of phosphatidylcholine (PC) vesicles are broadened by spin-labeled PC. The broadening of the N-methyl proton line is proportional to spin-labeled PC concentration; the broadening by 1 molecule in 100 of spin-labeled PC is 9.7 Hz at 35{degrees}C; the broadening is not due to collisions between vesicles, exchange of spin label between vesicles, or fusion of vesicles. These findings are proof of rapid diffusion in the plane of the PC bilayer (lateral diffusion). A further study of the broadening reveals the molecular frequency of the translation step for lateral diffusion to be much greater than 3 x 103 sec-1 at 0{degrees}C.
关键词：spin-labeled phosphatidylcholine ; nuclear resonance