文章基本信息

标题：Characterization of the Inducer Required for the Development of Macrophage and Granulocyte Colonies
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作者：Tamar Landau ; Leo Sachs
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1971
卷号：68
期号：10
页码：2540-2544
DOI：10.1073/pnas.68.10.2540
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：A substance produced by various types of cells can induce from single hematopoietic cells the formation of colonies of normal macrophages and granulocytes. This inducer has been purified 600-fold from serum-free conditioned medium from a tissue culture line of mouse cells. It is shown that the inducer is a protein, with a molecular weight of 65-70,000, whose inducing activity was about 1 ng per colony. Acrylamide gel electrophoresis of this material gave four bands, only one of which contained inducing activity. The purified protein was inactive. Activity was regained by the addition of a low molecular weight cofactor that is present in conditioned medium. Under certain conditions, activity was also regained by the addition of adenosine 3':5'-cyclic monophosphate (cyclic AMP).
关键词：hematopoietic ; cofactor ; cyclic AMP ; ultrafiltration ; protein inducer