文章基本信息

标题：Enhanced Release of Dopamine-β-Hydroxylase from Sympathetic Nerves by Calcium and Phenoxybenzamine and Its Reversal by Prostaglandins
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作者：David G. Johnson ; Nguyen B. Thoa ; Richard Weinshilboum 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1971
卷号：68
期号：9
页码：2227-2230
DOI：10.1073/pnas.68.9.2227
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Dopamine-{beta}-hydroxylase (EC 1.14.2.1 ), an enzyme localized in sympathetic synaptic vesicles, was released together with norepinephrine during in vitro stimulation of the hypogastric nerve that innervates the vas deferens of the guinea pig. Stimulation of the nerve in the presence of high concentrations of calcium (7.5 mM) or phenoxybenzamine caused a marked increase in the amounts of the enzyme and norepinephrine released into the bath. The augmentation of release of dopamine-{beta}-hydroxylase by 7.5 mM calcium or phenoxybenzamine was reversed by prostaglandin E2. These findings suggest that the release of the sympathetic neurotransmitter, norepinephrine, occurs by a process of exocytosis in which the vesicular content of soluble dopamine-{beta}-hydroxylase is also released. The depolarization-induced exocytosis, which is stimulated by calcium, may be affected by prostaglandin E2 or phenoxybenzamine through inhibition or enhancement of the actions of calcium in the release process.
关键词：norepinephrine ; hypogastric nerves ; vas deferens ; guinea pigs