文章基本信息

标题：Cell Fractionation and Arrangement on Fibers, Beads, and Surfaces
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作者：G. M. Edelman ; U. Rutishauser ; C. F. Millette 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1971
卷号：68
期号：9
页码：2153-2157
DOI：10.1073/pnas.68.9.2153
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：A new method, fiber fractionation, has been used to isolate and separate cells. The cells are adsorbed to fibers covalently coupled to molecules such as antigens, antibodies, and lectins which can bind specifically to cell-surface components. The cells are then removed mechanically by plucking the taut fibers. Alternatively, competitive inhibitors of binding may be used to remove the cells at a lesser rate. Successful fractionations have been achieved by varying the degree of derivatization of the fibers by the lectin concanavalin A. Lymphoid cells have been separated by the use of different antigens coupled to the fibers. The method may also be used for specific fixation and manipulation of viable cell populations in culture. In addition to fibers, beads and surfaces have been specifically derivatized and used to achieve different geometrical arrangements of the cells.
关键词：immunology ; cell culture ; lectins ; concanavalin A ; erythrocytes