文章基本信息

标题：Purification and In Vitro Transcription of a Transfer RNA Gene
本地全文：下载
作者：Violet Daniel ; Jacques S. Beckmann ; Sara Sarid 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1971
卷号：68
期号：9
页码：2268-2272
DOI：10.1073/pnas.68.9.2268
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：A gene specifying tyrosine transfer RNA has been purified and transcribed in vitro. The purification procedure made use of two specialized transducing phages carrying the tRNATyr gene of Escherichia coli inserted into their DNA in opposite orientations. The separated heavy strands of the two phages were annealed and the single-stranded tails of the resulting hybrid were removed by digestion with Neurospora endonuclease. The size of the purified double-stranded structures was determined by electron microscopy. These isolated duplexes served as template for the in vitro transcription of tRNATyr-like molecules.
关键词：E. coli ; bacteriophage ; Neurospora endonuclease ; tyrosine ; electron microscopy