文章基本信息

标题：DNA-Directed Cell-Free Synthesis of Biologically Active Transfer RNA: su+III Tyrosyl-tRNA
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作者：Geoffrey Zubay ; Loretta Cheong ; Malcolm Gefter 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1971
卷号：68
期号：9
页码：2195-2197
DOI：10.1073/pnas.68.9.2195
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Biologically active su+III tyrosyl-tRNA has been synthesized in a DNA-directed cell-free system from Escerichia coli. Such a system should be most useful for studying the mechanism of tRNA synthesis. This tRNA is capable of suppressing amber mutations in the gene coding for {beta}-galactosidase (EC 3.2.1.23 ) and, therefore, must be capable of being charged and transferring amino acids. A 4-fold stimulation in the activity of the tRNA formed de novo is obtained with isopentenyl pyrophosphate, a compound involved in the post-transcriptional acylation of an adenine base adjacent to the anticodon. It has been suggested elsewhere that formation of RNA subject to stringent control may be inhibited by guanosine tetraphosphate (ppGpp). However, guanosine tetraphosphate did not affect the synthesis of su+III tyrosyl-tRNA, even though the synthesis of this tRNA is subject to stringent control.
关键词：E. coli ; β-galactosidase ; bacteriophage