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  • 标题:Inhibition by Siomycin and Thiostrepton of Both Aminoacyl-tRNA and Factor G Binding to Ribosomes
  • 本地全文:下载
  • 作者:Juan Modole Ll ; Bartolomé Cabrer ; Andrea Parmeggiani
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:1971
  • 卷号:68
  • 期号:8
  • 页码:1796-1800
  • DOI:10.1073/pnas.68.8.1796
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:Siomycin, a peptide antibiotic that interacts with the 50S ribosomal subunit and inhibits binding of factor G, is shown also to inhibit binding of aminoacyl-tRNA; however, it does not impair binding of fMet-tRNA and completion of the initiation complex. Moreover, unlike other inhibitors of aminoacyl-tRNA binding (tetracycline, sparsomycin, and streptogramin A), siomycin completely abolishes the GTPase activity associated with the binding of aminoacyl-tRNA catalyzed by factor Tu. A single-site interaction of siomycin appears to be responsible for its effect on both the binding of the aminoacyl-tRNA-Tu-GTP complex and that of factor G.
  • 关键词:Millipore filter ; Tu ; tetracycline ; streptogramin ; puromycin
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