文章基本信息

标题：Acetyl CoA Carboxylase: Isolation and Characterization of Native Biotin Carboxyl Carrier Protein
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作者：R. Ray Fall ; A. M. Nervi ; Alfred W. Alberts 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1971
卷号：68
期号：7
页码：1512-1515
DOI：10.1073/pnas.68.7.1512
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：A large form of biotin carboxyl carrier protein (BCCPL) has been isolated from extracts of Escherichia coli. It has a minimal molecular weight of 20,000, according to its behavior on sodium dodecylsulfate-polyacrylamide gel electrophoresis, and contains approximately 1 mol of biotin per 22,000 g of protein. BCCPL exhibits Km values, in the biotin carboxylase and transcarboxylase half-reactions of acetyl CoA carboxylase, of 2 x 10-7 M and 4 x 10-7 M, respectively; these values are 50-100 times lower than those obtained with smaller forms of BCCP previously isolated. Electrophoresis of crude extracts of E. coli indicates that the major biotin-containing protein migrates at the same rate as BCCPL, which suggests that BCCPL is the native form of BCCP in E. coli.
关键词：high molecular weight ; high activity