文章基本信息

标题：A Bovine Pancreatic Enzyme Catalyzing the Conversion of Proinsulin to Insulin
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作者：C. C. Yip
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1971
卷号：68
期号：6
页码：1312-1315
DOI：10.1073/pnas.68.6.1312
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：An enzyme that catalyzes the conversion of bovine proinsulin to insulin has been purified from a bovine pancreatic extract. The product of conversion was identified as insulin by aminoacid analysis and determination of carboxyl terminal aminoacid residues. The purified enzyme preparation showed one major protein band on polyacrylamide gel disc electrophoresis; it had a molecular weight of about 70,000 and an isoelectric point (pI) at a pH of 4.82.
关键词：amino acid analysis ; gel electrophoresis ; tryptic activity ; molecular weight