文章基本信息

标题：Adenosine Triphosphate-Dependent Synthesis of Biologically Active DNA by Azide-Poisoned Bacteria
本地全文：下载
作者：A. T. Ganesan
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1971
卷号：68
期号：6
页码：1296-1300
DOI：10.1073/pnas.68.6.1296
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Nonviable cells of Bacillus subtilis, when made permeable by treatment under controlled conditions with nonionic detergent, can be shown to replicate DNA normally and to repair certain regions of the chromosome. The former process is stimulated by ATP in the presence of dATP, dCTP, dGTP, and dTTP. The product, a result of semiconservative replication, is biologically active; synthesis of the newly-formed regions of the chromosome appears to be sequential.
关键词：Bacillus subtilis ; gene markers ; detergent Brij-58