文章基本信息

标题：Heterogeneity, Complexity, and Repetition of the Chloroplast DNA of Chlamydomonas reinhardtii
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作者：Deepak Bastia ; Kwen-Sheng Chiang ; Hewson Swift 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1971
卷号：68
期号：6
页码：1157-1161
DOI：10.1073/pnas.68.6.1157
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The chloroplast DNA of wild-type Chlamydomonas reinhardtii was isolated in a CsCl density gradient as a single, homogeneous density class with a mean density of 1.695 g/cm3. Irrespective of sheared size, denatured chloroplast DNA renatured as a single homogeneous species. Compositional heterogeneity, presumably intramolecular, was revealed by the absorbance-temperature profile. The complement of unique nucleotide sequences of the chloroplast DNA, as determined by the rate of renaturation, was 1.94 x 108 daltons. This kinetic complexity is 26-fold less than the DNA content of a single gamete chloroplast, and 52-fold less than the chloroplast of a vegetative cell, which indicates that the chloroplast of C. reinhardtii possesses at least 26 copies of a unique nucleotide sequence.
关键词：T_m ; density gradient centrifugation ; reannealing ; hydroxyapatite chromatography