文章基本信息

标题：Quantitative In Vitro Measurement of Simian Virus 40 Tumor-Specific Antigens
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作者：Peter W. Wright ; Lloyd W. Law
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1971
卷号：68
期号：5
页码：973-976
DOI：10.1073/pnas.68.5.973
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Humoral antibodies, specific for the SV40 tumor-specific transplantation antigen, can be detected by an in vitro cytotoxic assay. 51Cr-labeled SV40-transformed target cells are selectively killed in the presence of specific antibody and rabbit complement. Inhibition of cytotoxicity can be used to measure the antigen concentration and specific activity on different SV40-transformed cell lines. The assay described has several advantages when compared to other methods currently available to detect the specific SV40 antigen: it is simple, rapid, objective, reproducible, and quantitative.