文章基本信息

标题：The Reovirus Replicative Cycle: Conservation of Parental RNA and Protein
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作者：Samuel C. Silverstein ; Michael Schonberg ; Daniel H. Levin 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1970
卷号：67
期号：1
页码：275-281
DOI：10.1073/pnas.67.1.275
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The fate of parental reovirions in the viral replicative cycle has been analyzed using CsCl density centrifugation. After penetration of L-cells, reovirus is converted from a particle of density 1.39 g/cm3 to a subviral particle of density 1.41 g/cm3. This alteration in density is temporally correlated with the hydrolysis of viral coat proteins and is qualitatively similar when particles are labeled in their RNA or protein. Ten hours after infection, when synthesis of progeny virus is underway, the parental RNA and protein are again found at density 1.39 g/cm3. These data demonstrate conservation of the parental RNA and protein in the subviral particle throughout the replicative cycle.