文章基本信息

标题：Genetically Induced Subcellular Mislocation of Neurospora Mitochondrial Malate Dehydrogenase
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作者：K. D. Munkres ; K. Benveniste ; J. Gorski 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1970
卷号：67
期号：1
页码：263-270
DOI：10.1073/pnas.67.1.263
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Among 60 ultraviolet-induced missense mutations of the structural genes that code for mitochondrial malate dehydrogenase (M-MDH, EC 1.1.1.37 ) of Neurospora crassa, two enzyme phenotypes are observed. In a previously described class (C-mutants), M-MDH is malfunctional because of an abnormal conformation induced by association with mitochondria. We describe here a second class (K-mutants) in which the enzyme is malfunctional because of an altered subcellular location. Thus, although both classes cause lesions in the assimilation of exogenous malate, the nature of the lesions differs. In C-mutants, the enzyme misfunctions because of low affinity for malate but remains mitochondrial-bound as in wild-type. Conversely, K-mutant M-MDH is dispersed throughout the cytoplasm. Studies of a repressible "glyoxysome" isozyme and a constitutive M-MDH of prototroph and mutants indicate that both isozymes are encoded by the same nuclear structural genes and have polypeptide subunits in common.