文章基本信息

标题：In Vitro TRANSCRIPTION OF A TRANSFER RNA GENE
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作者：Violet Daniel ; Sara Sarid ; Jacques S. Beckmann 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1970
卷号：66
期号：4
页码：1260-1266
DOI：10.1073/pnas.66.4.1260
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The Escherichia coli tRNATyr gene carried by the {phi}80psu3 + transducing phage was transcribed in vitro by DNA-dependent RNA polymerase. The enzymatically synthesized tRNATyr-like polynucleotide chains were detected by competition with purified E. coli32P-tRNATyr for specific hybridization sites on {phi}80psu3 + DNA. Analysis by sucrose gradient centrifugation showed the tRNATyr-like chains to possess a heterogeneous distribution with respect to sedimentation coefficients, with a broad peak around 8 S. The presence of the termination factor {rho} during the transcription did not significantly reduce the average size of the in vitro synthesized tRNATyr-like chains.