文章基本信息

标题：Dissection of functional domains of adenovirus DNA polymerase by linker-insertion mutagenesis
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作者：M Chen ; M S Horwitz
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1989
卷号：86
期号：16
页码：6116-6120
DOI：10.1073/pnas.86.16.6116
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Linker-insertion mutations were introduced into the cloned adenovirus DNA polymerase gene and the functional effects on the initiation and elongation of DNA in vitro were measured. Essential regions of the polymerase appear to be scattered in patches across the entire molecule and are not limited to the five regions of homology shared with a variety of other replicating polymerases. Thus, the adenovirus DNA polymerase presumably contains active sites that must be formed by distant interactions across the polymerase molecule.