文章基本信息

标题：Stimulation of human granulocyte function by monoclonal antibody WEM-G1
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作者：A F López ; M A Vadas
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1984
卷号：81
期号：6
页码：1818-1821
DOI：10.1073/pnas.81.6.1818
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：From a panel of monoclonal antibodies (MAb) produced against purified human neutrophils, MAb WEM-G1 was selected for its ability to stimulate granulocyte function as assessed by the capacity to kill antibody-coated tumor target cells. MAb WEM-G1 bound to neutrophils and eosinophils and not to monocytes, lymphocytes, or erythrocytes, and thus identified a granulocyte differentiation antigen. It enhanced killing by both neutrophils and eosinophils in a dose-dependent fashion, with a range of effector-to-target ratios and dilutions of anti-target cell antibody. The effect of MAb WEM-G1 was additive with that of colony-stimulating factor (CSF-alpha). Similarly, the ability of neutrophils to exhibit enhanced cytotoxicity in the presence of WEM-G1 was increased by preincubation of neutrophils with CSF-alpha. Immunoprecipitation analysis showed that WEM-G1 identified a cell-surface protein on human neutrophils of approximate molecular weight 110,000. It is suggested that this structure is involved in regulation of the function of human granulocytes.