文章基本信息

标题：Biogenesis of 3-hydroxy-3-methylglutaryl-coenzyme A reductase, an integral glycoprotein of the endoplasmic reticulum
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作者：D A Brown ; R D Simoni
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1984
卷号：81
期号：6
页码：1674-1678
DOI：10.1073/pnas.81.6.1674
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Using a cell line, C100, that overproduces 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMG-CoA reductase; EC 1.1.1.34 ) 100-fold, we have studied the synthesis and insertion of this protein into the endoplasmic reticulum. The enzyme is synthesized on membrane-bound polysomes. It is cotranslationally but not post-translationally inserted into dog pancreatic microsomes. This cotranslational insertion is dependent upon signal recognition particle. HMG-CoA reductase is glycosylated with an oligosaccharide(s) of the "high-mannose" type sensitive to endo-beta-D-N-acetylglucosaminidase H. Partial determination of the NH2-terminal amino acid sequence of the in vitro translation product and the mature polypeptide indicate they are the same and demonstrate there is no cleavage of an NH2-terminal signal sequence.