文章基本信息

标题：Total synthesis of insulin-like growth factor I (somatomedin C)
本地全文：下载
作者：C H Li ; D Yamashiro ; D Gospodarowicz 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1983
卷号：80
期号：8
页码：2216-2220
DOI：10.1073/pnas.80.8.2216
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Human insulin-like growth factor I (somatomedin C) with 70 amino acid residues and three disulfide bridges has been synthesized by the solid-phase method. The synthetic product behaves as a pure polypeptide in paper electrophoresis, isoelectric focusing, and high-performance liquid chromatography, Its elution behavior in Sephadex G-50, isoelectric point, amino acid composition, and growth-stimulating activity in bovine adrenal cortical cells or granulosa cells are comparable to those reported for the natural product. In radioimmunoassay, the synthetic product is indistinguishable from the natural hormone when either synthetic or natural product is used as the labeled ligand.