文章基本信息

标题：Procedure for rapid isolation of photosynthetic reaction centers using cytochrome c affinity chromatography
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作者：G. W. Brudvig ; S. T. Worland ; K. Sauer 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1983
卷号：80
期号：3
页码：683-686
DOI：10.1073/pnas.80.3.683
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Horse heart cytochrome c linked to Sepharose 4B is used to purify reaction centers from Rhodopseudomonas sphaeroides R-26. This procedure allows for an initial recovery of 80-90% of the bacterial reaction centers present in chromatophore membranes. High purity reaction centers (A280/A802 < 1.30) can be obtained with a 30% recovery. Reaction centers from wild-type Rps. sphaeroides and Rps. capsulata also bind to a cytochrome c column. Cytochrome c affinity chromatography can also be used to isolate photosystem I complexes from spinach chloroplasts.
关键词：photosynthetic bacteria ; spinach chloroplast ; membrane protein purification ; detergent solubilization