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标题：Expression of the beta subunit of spectrin in nonerythroid cells
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作者：W J Nelson ; E Lazarides
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1983
卷号：80
期号：2
页码：363-367
DOI：10.1073/pnas.80.2.363
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Antibodies raised against electrophoretically purified chicken erythrocyte beta subunit of spectrin, called "beta-spectrin," have been used to demonstrate the presence of an immunoreactive form of this polypeptide in nonerythroid tissues. Immunoautoradiography shows that, in chicken erythrocytes, this antiserum reacts with beta-spectrin (Mr 220,000) and another polypeptide (Mr 230,000) that, by two-dimensional tryptic peptide analysis, shows extensive homology with beta-spectrin but not with the alpha subunit of spectrin, called "alpha-spectrin." Immunoautoradiography and immunoprecipitation of various chicken tissues with this antiserum shows that either one variant or both variants of beta-spectrin are expressed. Indirect immunofluorescence reveals that the antiserum reacts with a plasma membrane-associated component of erythroid and some nonerythroid cells. Particularly strong fluorescence is observed in skeletal and cardiac muscle cells where beta-spectrin appears to form a grid-like network along the inner surface of the sarcolemma. The noncoordinated distribution of alpha- and beta-spectrin variants indicates that their expression may be tailored to the functional requirements of the plasma membrane in different cells.