文章基本信息

标题：Properties of a cell-free system for synthesis of citrus exocortis viroid
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作者：Ricardo Flores ; J. S. Semancik
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1982
卷号：79
期号：20
页码：6285-6288
DOI：10.1073/pnas.79.20.6285
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Partially purified nuclei from citrus exocortis viroid (CEV)-infected Gynura aurantiaca are able to synthesize linear and circular viroid molecules. Pretreatment of the nuclei with actinomycin D or deoxyribonuclease did not affect viroid synthesis, whereas the synthesis of other cellular RNAs was severely reduced. These observations support the essential role of CEV complementary RNA sequences in viroid replication. However, when -amanitin was included in this in vitro synthesis system, CEV replication was markedly reduced by concentrations of 10 nM or greater. Taken together, these data support the proposition that viroid synthesis is catalyzed by a DNA-dependent RNA polymerase acting on a RNA template.
关键词：nuclei-rich fraction ; actinomycin D ; DNase ; α-amanitin ; denaturing gel