文章基本信息

标题：Structures of the genes for the β and ε subunits of spinach chloroplast ATPase indicate a dicistronic mRNA and an overlapping translation stop/start signal
本地全文：下载
作者：Gerard Zurawski ; Warwick Bottomley ; Paul R. Whitfeld 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1982
卷号：79
期号：20
页码：6260-6264
DOI：10.1073/pnas.79.20.6260
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：A 2,4-kilobase-pair region of spinach chloroplast DNA adjacent to the gene for the large subunit of ribulosebisphosphate carboxylase has been analyzed by RNA hybridization, in vitro transcription/translation, and DNA sequence determination. The analysis indicates that this region carries the genes for the {beta} and {varepsilon} subunits of chloroplast ATPase and that the two genes are cotranscribed into a dicistronic mRNA with 4-base-pair overlap between the stop codon of the {beta}-subunit gene and the start codon of the {varepsilon}-subunit gene. The ATPase and carboxylase genes are transcribed divergently with respect to each other. The deduced amino acid sequences of the {beta} and {varepsilon} subunits from spinach show 67% and 26% homology, respectively, with the published sequences of the {beta} and {varepsilon} subunits of Escherichia coli ATPase.
关键词：chloroplast DNA ; nucleotide sequence ; amino acid sequence ; homology with Escherichia coli ATPase ; overlapping genes