文章基本信息

标题：Aluminum: a requirement for activation of the regulatory component of adenylate cyclase by fluoride
本地全文：下载
作者：P C Sternweis ; A G Gilman
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1982
卷号：79
期号：16
页码：4888-4891
DOI：10.1073/pnas.79.16.4888
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Activation of the purified guanine nucleotide-binding regulatory component (G/F) of adenylate cyclase by F- requires the presence of Mg2+ and another factor. This factor, which contaminates commercial preparations of various nucleotides and disposable glass test tubes, has been identified as Al3+. In the presence of 10 mM Mg2+ and 5 mM F-, AlCl3 causes activation of G/F with an apparent activation constant of approximately 1-5 muM. The requirement for Al3+ is highly specific; of 28 other metals tested, only Be2+ promoted activation of G/F by F-.